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An In Vitro Analysis Of Natural And Allopathic Antimicrobial Agents Onto Guided Tissue Regeneration Against Periodontal Pathogens 
Parth Mehra1*, Amrita2
Keywords
Tetracyclines, GTR membrane, Zones of inhibition
Abstract
Background
It is well established that plaque control is a critical determinant of the success or failure of various
outcomes of periodontal therapy.
Materials and Methods
Tetracyclines in their purest form were obtained for the investigation. Pure green coffee extract, which
contains 50% Chlorogenic Acid (CGA), was purchased from Top Secret Nutrition Products. Following microbial analysis, the producer confirmed that it was free of all types of bacteria, yeast, and mould. For each bacterium, the microbiological process was carried out three times. GTR membrane
treated with distilled water served as the research's control. SPSS 21.0 software was utilized for the
study's statistical analysis. The Kruskal Wallis ANOVA and Mann-Whitney U test were used to collect and analyze the statistical data.
Results
Zones of inhibition of Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and
Porphyromonas gingivalis (P. gingivalis) by 1% tetracycline, 1% metronidazole, 1% green coffee extract, and distilled water. With regard to both bacterial species, distilled water and green coffee extract displayed no zone of inhibition. While 1% tetracycline showed the broadest zone of inhibition across both A. actinomycetemcomitans and P. gingivalis. The mean zone of inhibition for each bacterium for each antimicrobial agent used in the research was determined for assessment.
Conclusion
The number of periodontal bacteria colonizing the GTR membrane can be efficiently re duced by
using readily available antimicrobial medications in such a low concentration.
How to cite this article:
Parth Mehra1*, Amrita,
Post Graduate Student, Department of Periodontology and Implantology, Institute of Dental Sciences, India. Journal of Clinical Trails & Research, 2023; 1(1): 20-23.
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